The present work aimed to investigate the protective effect of L-theanine on cognitive impairment induced by chronic restraint stress (CRS) in mice. Stress was induced by restraining animals in well-ventilated polypropylene tubes (3.2 cm diameter × 10.5 cm length) for 8 h once daily for 21 consecutive days.
L-theanine (2 and 4 mg/kg) was administered 30 min before animals were subjected to acute immobilized stress. At week 4, mice were subjected to a Morris water maze and step-through tests to measure cognitive function, followed by oxidative parameters and then corticosterone and catecholamines (norepinephrine and dopamine).
Our results showed that cognitive performance was significantly deteriorated in the CRS group, accompanied by noticeable changes in oxidative parameters and catecholamine levels in the hippocampus and cerebral cortex, as well as serum corticosterone and catecholamine levels. However, L-theanine treatment showed not only reversal of CRS-induced cognitive impairment and oxidative damage, but also reversal of abnormal serum corticosterone levels and abnormal brain and serum catecholamine levels.
This study indicated the protective effect of L-theanine against CRS-induced cognitive impairment in mice.
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