We investigated the effects of orally administered glycine on myofibrillar proteolysis in food-deficient chicks. Feed-deprived chicks (24 h) were orally administered 57, 113, and 225 mg glycine/100 g body weight and killed after 2 h.
Plasma N(tau)-methylhistidine concentration, used as myofibrillar proteolysis, was decreased by glycine. We also examined the expression of proteolytically related genes by real-time PCR of cDNA from chick skeletal muscle. The mRNA expression of atrogin-1/MAFbx, proteasome C2 subunit, m-calpain large subunit, and cathepsin B was decreased by glycine in a dose-dependent manner.
Plasma corticosterone concentration was also decreased by glycine, but plasma insulin concentration was unaffected. These results suggest that orally administered glycine suppresses myofibrillar proteolysis and expression of proteolytically related skeletal muscle genes by decreasing plasma corticosterone concentration in chicks.
Found at Alkohol adé (german)
